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LH is the main tropic regulator of Leydig cell function without which androgen production is not possible.[14] The observed decrease in serum testosterone level of rats treated with the two doses of nicotine is not associated with a decrease in LH thus suggesting that the etiology is not associated with a disorder in the pituitary but rather a testicular dysfunction, which is indicated when low serum testosterone levels are accompanied by high levels of serum LH