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To assess the function of each mitochondrial complex, O 2 consumption was assessed with the addition of a series of respiratory substrates and inhibitors in the following order and final concentrations in the chamber: glutamate-malate (10 and 2 mM), ADP (5 mM), octanoyl-carnitine (1.5 mM), succinate (10 mM), cytochrome c (10 M), rotenone (0.5 M), antimycin-A (2.5 M), oligomycin (2 M), and N , N , N , N -tetramethyl- p -phenylenediamine (TMPD)-ascorbate (0.5 and 2.0 mM)