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Incapsula incident ID: 229000720174633012-15743103854252548

Enzyme-linked immunosorbent assay (ELISA) To confirm hepatic levels of pro-inflammatory cytokines, such as tumor necrosis factor alpha (TNF) and interleukin 1 beta (IL1), protein samples were prepared and measured using ELISA kits (Invitrogen, CA, United States) in accordance with the manufacturers protocol

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Interestingly, the most common reason middle and high school students give for using e-cigarettes is feeling anxious, stressed, or depressed. This suggests many young people are self-medicating with nicotine without understanding how it can ultimately make mental health struggles worse