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At this time, microorganisms with cellulolytic and hemicellulolytic abilities (e.g., Aspergillus , Bacillus ) become the dominant groups, which continue to degrade macromolecular substances and provide nutrients for other microorganisms ( Nitrogen sources in cigar tobacco leaves include protein, amino acid, nicotine and other nitrogen-containing compounds, among which nicotine is a unique nitrogen source and carbon source in cigar tobacco leaves, and is the core nutritional factor driving the assembly of nicotine-degrading microbial communities (Meng et al., 2010

Meanwhile, according to a 2023 study by the National Institute on Drug Abuse, the percentage of young adults (ages 19-30), who reported past-year marijuana use and daily marijuana use reached their highest levels ever

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