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Nicotine also increases the production of DHT (Dihydrotestosterone), a hormone that contributes to hair loss and prevents healthy hair growth

While the importance of this change in surfactant structure is still unknown to us, this observation suggests that slight changes in surfactant do occur after exposure to e-cigarette and might result in changes in surface tension if a higher dose of e-cigarette vapor is used

Direct Answer: No puff count alone does not reliably indicate nicotine intake

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