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Step 5: Seal and Finish Once your cigarette is neatly rolled, moisten the adhesive strip on the rolling paper and seal it shut

Extracellular flux assayglycolysis rate assay Cells were plated at a density of 7500 cells/well in XF96 micro-plates (Agilent Technologies) in DMEM/F12 supplemented with 10% FBS 48 h prior to the assay and cultivated in a humified incubator at 37 C with 5% CO 2

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When they attacked their hosts, the well-prepared Ngi Tahu killed all the leading Ngti Toa chiefs except Te Rauparaha, who returned to his Kapiti Island stronghold