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iCLIP-seq Cell collection and iCLIP-seq protocol In total, 2.5 10 7 HeLa-S3-FlpIn control and BCDIN3D-KO cells grown in spinner flasks were collected by centrifugation at 200 g for 5 min at 4 C, and washed once with 25 mL of cold 1 PBS

The MEA region is characterized by high unmet demand for smoking cessation aids in markets such as Saudi Arabia, the UAE, Turkey, and South Africa, where combustible cigarette prevalence remains elevated and healthcare infrastructure is increasingly directing resources toward cessation programs