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Studies distinguishing between e-cigarettes with and without nicotine found increases in endothelial progenitor cells, endothelial vesicles, plasma myeloperoxidase, arterial stiffness, changes in nicotine receptors, alveolar macrophages, and mononuclear phagocytes, leading to altered inflammatory responses and increased pulmonary susceptibility in users of nicotine-containing e-cigarettes (29, 41, 46)

If its within 30 minutes, its moderate, and if its within 60 minutes or later, its somewhat lower

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