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Maternal smoking is associated with several complications of pregnancy including abruption placentae, placenta previa, bleeding during pregnancy, premature and prolonged rupture of the membranes, and preterm delivery

The bulk of the problem is that many smokers who wouldnt dream of throwing their food wrappers or drink cups out the window think nothing of dropping their cigarette butts on the ground wherever they are

Melting curve analysis was performed to evaluate the purity of the PCR products