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Cell pellets resuspended in BEGM + 0.5 PSA were filtered using a 70 m cell strainer, collected by centrifugation (5 min, 225 g , 4 C) and cryopreserved in freeze media (F-media, 30% FBS, 10% DMSO)

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Interestingly, a study investigating sub-chronic exposure to carbon nanotube (CNT) particles, cigarette smoke extract (CSE), and their combination on lung microbiota found that CSE altered lung microbiota, shifting from Proteobacteria to Bacteroidetes, while co-exposure with CNT resulted in mixed microbial effects, including increased Bacteroidetes and Tenericutes