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Multiple in vitro and in vivo studies have confirmed nicotine's direct effect on specific inflammatory mediators: TNF- : Nicotine suppresses TNF- production from lipopolysaccharide (LPS)-stimulated macrophages the same target as blockbuster anti-inflammatory biologics like adalimumab (Humira) IL-1 and IL-6 : Both significantly reduced in studies of nicotine-treated macrophage cultures IL-2 and IL-8 : Reduced synthesis by mononuclear cells in nicotine-exposed models relevant to inflammatory bowel disease NF-B activity : Nicotine directly inhibits NF-B nuclear translocation in multiple cell lines, preventing the inflammatory gene expression cascade from starting What's particularly striking is that these are the same markers that define chronic systemic inflammation the kind linked to cardiovascular disease, metabolic syndrome, and autoimmune conditions

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