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Multigenic DNA constructs were created using DNA parts from GoldenBraid 2.0 kit, from Diego Orzaez (Addgene kit # 1000000076, basic plasmids, P19 ORF, Nos terminator), MoClo Toolkit, from Sylvestre Marillonnet (Addgene kit # 1000000044, 35S promoter and reporter genes), and using parts prepared at Laboratory of Virology IEB, Prague (MAR sequences, extended alpha 1114 entry plasmids, domestication of pEAQ derived 5/3-UTR regions) and in the BRU, Cape Town (domesticated BeYDV sequences)

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