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If 150 heat cycles were performed, chromium and iron from Kanthal coils would be lost up to 19% and 58%, respectively, and iron and nickel from nichrome coils up to 14% and 43%, respectively [58]

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For the phosphorylated proteins, 1,000 l each of SuperSignal West Femto Luminol/Enhancer Solution (Thermo Scientific) and SuperSignal West Femto Stable Peroxide Buffer (Thermo Scientific) were mixed and pipetted onto the nitrocellulose membrane