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The PCR products were amplified with the ITS universal primer ITS 1:5-TCCGTAGGTGAACCTGCGG-3 and ITS 4:5-TCCTCCGCTTATTGATATGC-3

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The World Health Organization (WHO), US Centers for Disease Control and Prevention (CDC), and the Canadian Public Health Association (CPHA) initiated the Global Youth Tobacco Survey (GYTS) to provide detailed surveillance of youth tobacco use at 395 sites within 131 countries and the Gaza Strip and West Bank