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Liquid-suspended seed aliquots were vortexed and incubated for ~25 min in different concentrations of ethanol [70, 100, and 70% (v/v)], left to dry on sterile filter paper and placed on a sterile growth medium containing 0.5 Murashige and Skoog salts with vitamins, pH 5.7, 0.8% agar (w/v), and 1% (w/v) sucrose, if not stated otherwise (0% suc)

The root system was the main tissue for Cd accumulation, accounting for about 52% of all plant tissues on average, and the addition of two types of sulfur did not change the distribution pattern of Cd in all parts of the pepper

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