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Neuron-specific SQOR overexpression has been shown to decrease the volume of cerebral infarction after stroke ( 2 S with hydroxocobalamin protects mitochondrial function in ischemic brain tissue by preventing H 2 S from inhibiting Complex IV ( 2 S and generates thiolate anions, significantly alleviating ischemic brain injury in mice ( 2 S production has proven beneficial: for example, oral metronidazole (to eliminate H 2 S-producing gut microbes) or a low-sulfur diet reversed neuro-metabolic abnormalities in SQOR-deficient mice (Figure 3 illustrates the diverse roles of SQOR in several systemic diseases

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Depending on the plate layout, either LPS (1 g/ml) or ConA, (2.5 g/ml, Sigma-Aldrich, Steinheim, Germany) was added in four replicates per cow in addition to four unstimulated wells (with Seahorse medium for volume compensation)