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Another key issue in this line of research is the pharmacokinetic properties of LIR in rats, including absolute bioavailability and brain penetration, as well as typical side effects of the GLP-1 receptor agonist class, such as nausea

To help minimize these side effects, its recommended to start with a lower dose and gradually increase it over time

Both dynamics add to a challenging sustainability profile for chemical synthesis, raising the operating costs and shifting the calculus toward greener synthesis routes and / or recombinant expression

Materials and methods Reagents Bovine insulin was obtained from Sigma-Aldrich (St Louis, MO, USA) and dissolved in acidic water (pH 23) to make a stock solution