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At higher doses, some concern exists about pro-inflammatory effects: LL-37 can activate NF-kB pathways, potentially worsening certain inflammatory conditions
The serum is best applied to a clean, freshly washed scalp, ideally in the evening to allow the active peptide complex to work undisturbed overnight
Some people notice changes within a few weeks, while others take longer
The strongest human evidence is topical, including the Leyden 2002 photoaging trial and a 2023 IRB-approved 21-woman topical NEEL gel study reporting an average 28% increase in skin collagen density at 3 months
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