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Within 30 min, salivary cortisol levels reached levels more than twice as high as the control levels and remained elevated above control levels for more than 240 min (Fig

The subsequent series of adverse events include loss of membrane integrity, mitochondrial dysfunction, impaired sperm motility as well as DNA damage and apoptosis resulting in damage of cellular components and pathogenesis of reproductive diseases and infertility ( Thus, information obtained from the assessment of OS status could be of great importance in enhancing clinical decision making

2020;7(1):ofaa001

Subsequently, according to the method described in the previous study ( 2.7 Immunofluorescence (IF) After transcardial perfusion in mice, brain tissues were harvested and pretreated with gradient dehydration in 20% and 30% sucrose solutions at 4C (to reduce ice crystal damage), then embedded in optimal cutting temperature compound and sectioned for 20 m thickness using cryostat