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Samples were centrifuged at 3,000 g for 10 min at 4C to create a gradient, followed by removal of the top clear layer and middle debris-containing layer (45 ml)

Gut microbiota as a trigger for metabolic inflammation in obesity and type 2 diabetes

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Pathological high intraocular pressure (ph-IOP) is a critical feature in the development of glaucoma and a major factor leading to RGC loss [323]