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Previous studies have shown that the ROS content in oocytes subjected to aging in vitro is time-dependent [35]
Research-only context for DSIP in laboratory workflows DSIP is used in laboratory settings as a peptide reference input in controlled research workflows that may include analytical chemistry characterization, method development, stability assessments, compatibility checks with assay matrices, and in vitro assay development where a consistent and traceable compound identity is required
Subsequently, chloroplast proteins were extracted and incubated with about 50 g of purified protein or SCFE for 2 h at 25 C, then western blotting was performed
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