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ABHD11 purification To purify ABHD11-Flag protein, 1 10 8 HeLa cells expressing ABHD11-Flag were lysed in 1 ml lysis buffer (50 mM TrisHCl pH 7.4, 150 mM NaCl, 1% Triton X-100, 1 Roche complete protease inhibitor cocktail) and rotated at 4 C for 1 h

It lowers blood sugar by improving insulin sensitivity, decreasing the amount of blood sugar the liver produces, and reducing the amount of sugar the digestive system absorbs

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Komatsu, M., et al.,The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1