semaglutide how to inject Injection Sites: Abdomen vs. Thigh – Which Is Better? Injecting Semaglutide: A Guide to

Consult a healthcare provider for medical advice

1 Remove Melanotan I vial from refrigeration and allow to reach room temperature (1520 minutes) 2 Clean vial top with alcohol swab and allow to air dry completely 3 Using a sterile syringe, draw the calculated volume of bacteriostatic water (BAC water) 4 Inject BAC water slowly down the side of the vial do not aim directly at the powder 5 Gently swirl in circular motions DO NOT shake vigorously as this degrades the peptide 6 Allow 23 minutes for full dissolution solution should be clear and colorless 7 Draw calculated dose into an insulin syringe for subcutaneous injection 8 Inject into abdomen, thigh, or upper arm

Interpretation: It plays a critical role in reinforcing the gut barrier and supporting an anti-inflammatory environment

FISH analysis was performed as previously described 20 in buffer (20 mM Tris-HCl, 0.9 M NaCl, 0.01% SDS, 30% formamide) with DAPI and the Buchnera -specific probe ApisP2A-Cy5 (5-Cy5-CCTCTTTGGGTAGATCC-3)