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The bioavailability difference between fasted and fed injection can reach 30% due to gastric pH and competitive amino acid absorption

Tavares CAM, Azevedo LCP, Rea-Neto A, Campos NS, Amendola CP, Kozesinski-Nakatani AC, David-Joao PG, Lobo SM, Filiponi TC, Almeida GMB, Bergo RR, Guimaraes-Junior MRR, Figueiredo RC, Castro JR, Schuler CJ, Westphal GA, Carioca ACR, Monfradini F, Nieri J, Neves FMO, Paulo JA, Albuquerque CSN, Silva MCR, Kosiborod MN, Pereira AJ, Damiani LP, Correa TD, Serpa-Neto A, Berwanger O, Zampieri FG

Specifically, Ch2-Ch1 (B1-B2 map) RICS analysis of brightness (intensity of fluorescence signal) in one channel cross-correlates with brightness in the second channel, allowing for the calculation of cross-correlation