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Inject the bacteriostatic water slowly into the semaglutide vial, aiming the stream against the inner glass wall never directly at the powder

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Overall, the coverage of the protein was low, considering that 67% of the proteins showed equal or less than 10% of coverage

After inspection of the apo-OsDHAR structure, we predicted that the highly conserved Cys20 residue acts as the catalytic nucleophile and found that this residue is located at the cleft between the N- and C-terminal subdomains