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Expression of iron importers is upregulated in cancer and it correlates with tumor progression [14]

Then cells were harvested by centrifugation (6,000 rpm, 15 min), resuspended in lysis buffer [Tris-HCl (100 mM, pH 8.0), NaCl (300 mM), imidazole (20 mM), TCEP (5 mM), and PMSF (1 mM)], and incubated on ice with 1 mg/mL lysozyme for 30 min

The study incorporated DNAzymes into nanoliposomes to improve their ability to penetrate the BBB and reach cerebrovascular cells, thereby enhancing their therapeutic effectiveness for brain diseases

39 , 40 In both studies, the cytotoxicity of APR-246 was independent of the TP53 mutational status