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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

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[7] Men treated with finasteride should refrain from blood donation while taking finasteride

There are 2 strategies for testing glucose levels in early pregnancyusing the nonpregnancy-recommended screening tests (FPG or A1C) or using the typical 24- to 28-week gestational diabetes screening (50 g glucose challenge test [GCT] and/or 75 g oral glucose tolerance test [OGTT]) criteria (see below)