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These efforts involve chemically masking the secondary amine of MK-801 to render it inactive until the linker is cleaved, by the higher concentration of thiol-containing compounds in the intracellular compartment, resulting in liberation of MK-801 18

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(PubMed) Eshak ES, Iso H, Yamagishi K, Cui R, Tamakoshi A

This allows researchers to simulate true physiological spikes and observe authentic cell-autonomous recovery periods, eliminating the risks of premature pituitary fatigue or continuous receptor saturation during long-term in vitro assays