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doi: 10.3389/fmed.2026.1763185 Received 08 December 2025 Revised 20 January 2026 Accepted 26 January 2026 Published 12 February 2026 Volume 13 - 2026 Edited by Jianbin Zhang, Dalian Medical University, China Reviewed by Theodoros Androutsakos, National and Kapodistrian University of Athens, Greece Feng Zhang, Nanjing University of Chinese Medicine, China Updates Copyright 2026 Lu and Yang

Cardiomyocyte fractional shortening, calcium transients Adult mouse left ventricular cardiomyocytes (LVCMs) were loaded with 0.5 M Fura2-AM and placed on the stage of an inverted microscope in a chamber containing normal physiological Tyrodes solution (150 mM NaCl, 5.4 mM KCl, 1.2 mM MgCl 2 , 10 mM glucose, 2 mM Na-pyruvate, 1.8 mM CaCl 2 , and 5 mM HEPES, pH 7.35) 100