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Additionally, GO_CC analysis revealed that the downregulated proteins were not only enriched in extracellular/secreted proteins but also in cytoplasmic proteins, which aligns with the fact that many metabolism-associated proteins are primarily located in the cytoplasm

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capillaries form at higher densities in gray matter and the subfornical organ compared to white matter and the corpus callosum, venules possess looser BBB junctions than capillaries, and arterioles have lower ABCB1 transporter expression and thicker astrocytic perivascular sheaths 23,24

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