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The left hind paws were amputated and fixed in 4% PFA, then scanned it by microCT scanner (SkyScan 1176, Bruker, Belgium) using the following parameters: 35 m resolution, 85 kV, 385 A, 65 ms exposure time, 0.7 rotation step in 360, and a 1 mm Al filter

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Shaking creates shearing forces that can denature the peptide

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