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DFO, while effective in animal models, exhibits poor bloodbrain barrier (BBB) permeability, necessitating intranasal or intracerebroventricular delivery to achieve therapeutic concentrations (Guo et al., 2013)

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To test the possibility that Ex-4c may inhibit K ATP channels to activate POMC neurons, we pretreated the slices with 200 M tolbutamide, a K ATP channel blocker, and found that five of 12 cells (41.7%) were depolarized by 3.90.5 mV (from 48.01.7 to 44.11.4 mV, n =5) (Fig