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Then, the fixed cells were dehydrated in 60% isopropyl alcohol for 10 min, stained with filtered Oil Red O solution (60% Oil Red O stock solution/40% water) for 20 min and rinsed with 60% isopropanol

Ohnishi I, Iwashita Y, Matsushita Y, Ohtsuka S, Yamashita T, Inaba K, Fukazawa A, Ochiai H, Matsumoto K, Kurono N, Matsushima Y, Mori H, Suzuki S, Suzuki S, Tanioka F, Sugimura H (2021) Mass spectrometric profiling of DNA adducts in the human stomach associated with damage from environmental factors

Just like with topiramate, the medication is started at a low dose and increased gradually to help your body adjust and reduce the chance of side effects

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