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ArticlePubMedPMC 9

Whole-cell lysates were immunoprecipitated with anti-HA and then immunoblotted with anti-HA or anti-Flag

With the advancement of multi-omics sequencing technologies, including proteomics, transcriptomics, and metabolomics, alongside genetic code extension and machine learning, the mapping of lactylation networks, including lactylated proteins, specific modification sites, and protein function in various physiologies and pathologies, is anticipated to be elucidated more comprehensively