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Despite the flexibility within the N-terminal two residues, Glu3 of exendin-4 formed very similar contacts to that of Glu9 of GLP-1, however, interactions with residues residing at the base of the pocket were more transient for exendin-4 (7580% of the MD frames) than for GLP-1 in the MD simulations (97% of frames for Y152 1.47 and 100% for R190 2.60 ) (Supplementary Table 3)

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and (3) Wait 30 minutes before eating or taking any other medications

Missed or irregular doses can create fluctuations that impact both symptom control and lab values, making careful patient education critical for optimal outcomes