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Results and Regulatory Compliance The analytical workflow achieved: 100% sequence coverage, including modified residues. Accurate identification and localization of the lipidated Lys20 residue. Confirmation of structural integrity through complementary NMR analysis. The comprehensive data package, including validated methods, raw and processed spectra, and detailed reports, met the stringent requirements of regulatory agencies, facilitating the clients successful late-stage biologic filing. Conclusion This case study underscores ResolveMass Laboratories expertise in the peptide sequencing of Semaglutide, highlighting our capability to navigate complex analytical challenges and deliver data that withstands regulatory scrutiny