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To increase production across various agricultural settings, the breeding program has been flexible, employing techniques such as single-cross hybrid breeding, three-way cross, composite breeding, and double cross (Gong et al., 2015)

In brief, thiobarbituric acid (TBA) reagent (15% v/v trichloroacetic acid and 0.25 N HCl) was added to each sample (2:1), heated at 95 C for 15 min and allowed to cool

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