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The expression construct comprises tandem repeats of semaglutide main chain peptides 29 (Arg34GLP-1 (9-37)) fused with SNAC tags (GSHHW) and enterokinase recognition sites (DDDDK)

Example 14: Synthesis of peptide resin of Semaglutide [0078] The Fmoc-Gly-2-CTC resin synthesized in Example 6 was used for the synthesis of peptide resin of Semaglutide

[0068] Before the coupling of the Lys side chain, Alloc protecting groups were removed twice with a solution of 0.2 equivalents of Pd(PPh 3 ) 4 and 10 equivalents of morpholine in methylene chloride for 30 min each time

More Identification and expression of semaglutide main chain P29 with SNAC and DDDDK tags