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Refrigeration is a best practice to help maintain stability *within* the 28-day window, but it does not extend the expiration date
For protocols in the 1-2.5 mg range, 3 mL keeps injection volumes manageable at 10-25 units per dose
Two downstream consequences are mechanistically central: nicotinamide is preserved for re-entry into the NAD+ salvage pathway (raising intracellular NAD+ availability), and SAM is preserved for other methylation reactions
B12 is water soluble, which means B12 doesnt build up in your system once youre at the appropriate amount for your body you will just eliminate any extra, so theres very little risk of an overdose
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