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Specifically, GLP-1RAs exert protective effects on exercise-induced cardiomyopathy via increasing autophagy, reducing inflammation-related proteins and ROS generation via restoring the expression of catalase and manganese superoxide dismutase (MnSOD) [81]

Cells were incubated at 37 C for up to 5 minutes before aspirating the dissociation solution followed by addition of wash medium (N2B27) supplemented with 10 M ROCK inhibitor, 33 g /mL DNase I (Worthington), and 30 M actinomycin D

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