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Abbreviations ACN: Acetonitrile DMEM: Dulbeccos Modified Eagle Medium E/e: Ratio of early diastolic maximum transmitral inflow velocity (E) to early diastolic tissue velocity at the mitral valve annulus (e) ESI-MS/MS: Electrospray ionization tandem mass spectrometry FCS: Fetal calf serum FIA-MS/MS: Flow-injection MS/MS system FMO3: Flavin-containing mono-oxygenase 3 HBSS: Hanks balanced salt solution HF: Heart failure HFpEF: Heart failure with preserved ejection fraction LVEF: Left ventricular ejection fraction L-ZSF1: Lean ZSF1 rats MGV: Mean gray value MRM: Multiple reaction monitoring MS: Mass spectrometry NO: Nitric oxide NT-proBNP: N-terminal pro-brain natriuretic peptide OTU: Operational taxonomic unit O-ZSF1: Obese ZSF1 rats PAS: Periodic acid shift PBS: Phosphate-based saline ROI: Region of interest SDMA: Symmetric dimethylarginine TMA: Trimethylamine TMAO: Trimethylamine-N-oxide ZO1: Zona occludens protein 1 ZSF1: F1 hybrids crossed from Zucker diabetes fatty (ZDF)-rats with male spontaneously hypertensive heart failure (SHHF)-rats References McDonagh TA, Metra M, Adamo M, Gardner RS, Baumbach A, Bhm M, et al

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