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2.8 Assay of serum inflammatory markers and tissue oxidative indices markers 2.8.1 Determination of serum nitrite Nitrite was measured as an indicator of nitric oxide (NO) production according to the Griess method as described by Misco and other researchers ( 2.8.2 Enzyme-linked immunosorbent assay for determination of IL-1 and TNF- The Biolegend ELISA MAXTM Deluxe kit, USA, was utilized to measure the serum levels of TNF- and IL-1