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Mechanisms of action Preclinical studies document: Anxiolytic effect without sedation in classical tests (elevated plus maze, open field, light-dark box) GABAergic system modulation : allosteric potentiation of GABA-A observed ex vivo Enkephalin increase (endogenous opioid peptide) and serotonergic modulation Immunomodulatory effects inherited from tuftsin: macrophage activation, T-helper modulation Behavioural models Anxiolysis is measured via time spent in open arms of the elevated plus maze (EPM), visit ratio of central zones of open field , and exit latency in light-dark box

The reactions were initiated by adding ATP at a final concentration of 1 mM and incubated in a thermocycler (Bio-Rad, T100) at 30 C for 10 min

When that ratio drops, it signals increasing oxidative stress and cellular strain

He has also written for MindBodyGreen and About.com and given talks as part of the TEDx program