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This happens if the water was injected too rapidly (crushing the delicate peptide chains), the vial was shaken instead of gently swirled, or the solution's pH level is off

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Lymphocytes B level together with immunoglobulins G (IgG) and immunoglobulins A (IgA) showed a moderate correlation with age at blood drawn, IgG and immunoglobulins M (IgM) are associated with sex and IgG showed a moderate correlation with 2-macroglobulin levels ( Supplementary Tables 5 , 6 )

Science 2009;325:60710.PubMed 46