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Experimental studies indicate that calycosin may modulate the Toll-like receptor (TLR) 4/NF-B signaling pathway, suppress the expression of TSLP and IL-33, and increase tight junction proteins such as ZO-1 and occludin in both human immortalized keratinocyte cell line (HaCaT) and mouse AD models (Tao et al., 2017)

HUBERMAN: And by the way, that effect on increasing deep sleep, that non-REM sleep is something that's pretty well documented

For hair restoration specifically, some protocols combine subcutaneous GHK-Cu with direct scalp injection (mesotherapy) to deliver the peptide closer to the follicle bulb