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The cells were then fixed with frozen methanol for 15 min at 4 C and washed with PBS three times, followed by the addition of DAPI (4,6-diamidino-2-phenylindole, Beyotime Biotechnology Co

Correlational analysis between neurometabolite levels and clinical measures Gln levels in several regions were negatively correlated with the AASP Sensory Sensitivity and Sensation Avoiding subscale scores only in the ASD group (for details, see Fig