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In vivo , the enriched immunoreactivity or transcription of the GLP-1R was detected in neurons of the DVC, paraventricular and posterior thalamic nuclei, various hypothalamic regions, ventral, posterodorsal and interpeduncular tegmental areas, the periaqueductal gray and superior colliculus, while medium to low levels of GLP-1Rs were found in the posterior/caudal hippocampus, the hippocampal CA1, temporal/cerebral cortex, striatum, substantia nigra (SN) [which included tyrosine hydroxylase (SN)-expressing dopaminergic neurons (Elabi et al., 2021)], locus coeruleus, preoptic area, parabrachial nuclei, lateral septum, lateral habenula

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(3) heart rate within 10 beats of age-predicted maximum

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