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A normal response to the stimulus was first determined in the unoperated left hind limb and was defined as an immediate withdrawal of the limb with or without a vocalization

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Antibiotics with different mechanisms of action induce distinct patterns and kinetics of membrane potential changes: membrane-targeting antibiotics (e.g., polymyxins) typically trigger rapid and sharp depolarization, protein synthesis inhibitors (e.g., kanamycin) may cause a slower, more gradual depolarization, while nucleic acid inhibitors might show little early depolarization