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Briefly, RNA was isolated from whole-cell lysates using InviTrap Spin Universal RNA Mini Kit (Stratec) according to the manufacturers instructions

Reportedly, VEGF signaling increases VEGFR2 expression and phosphorylation, and stimulates proliferation in VEGF-stimulated C2C12 cells (Supplementary Figure S2), and this might potentially reduce the effects of lenvatinib and L-carnitine in C2C12 cells