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For Zepbound (weight management indication), doses increase from 2.5 mg to 5 mg, 7.5 mg, 10 mg, 12.5 mg, and 15 mg at 4-week intervals as tolerated

Briefly, a polyclonal rat anti-mouse LY-6G antibody (1:2000, BD Pharmingen 551459), a polyclonal rabbit anti-rat antibody (1:100, Vector AI-4001) and an envision anti-rabbit HRP (Dako K4003) were used, followed by a diaminobenzidine (Dako K3468)) to reveal the peroxidase activity, and then counterstained with hematoxylin

Step 3: Prepare for Potential Side Effects Since an early dose can cause a peak in medication levels, be proactive

The STEP 4 trial, which specifically examined continuation versus withdrawal of semaglutide, confirmed substantial weight regain in the discontinuation group